Papaya proteinase IV amino acid sequence

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Affinity purification of the novel cysteine proteinase papaya proteinase IV, and papain from papaya latex.

A procedure is described for the purification of a previously undetected cysteine proteinase, which we have called papaya proteinase IV, from spray-dried latex of the papaya (Carica papaya) plant. The purification involves affinity chromatography on Gly-Phe-aminoacetonitrile linked to CH-Sepharose 4B, with elution by 2-hydroxyethyl disulphide at pH 4.5. The product thus obtained is a mixture of...

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ژورنال

عنوان ژورنال: FEBS Letters

سال: 1989

ISSN: 0014-5793

DOI: 10.1016/0014-5793(89)81627-8